	   SnapGene      !�ACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCCTCGTTCATTCACGTTTTTGAACCCGTGGAGGACGGGCAGACTCGCGGTGCAAATGTGTTTTACAGCGTGATGGAGCAGATGAAGATGCTCGACACGCTGCAGAACACGCAGCTAGATTAACCCTAGAAAGATAATCATATTGTGACGTACGTTAAAGATAATCATGCGTAAAATTGACGCATGTGTTTTATCGGTCTGTATATCGAGGTTTATTTATTAATTTGAATAGATATTAAGTTTTATTATATTTACACTTACATACTAATAATAAATTCAACAAACAATTTATTTATGTTTATTTATTTATTAAAAAAAAACAAAAACTCAAAATTTCTTCTATAAAGTAACAAAACTTTTATGAGGGACAGCCCCCCCCCAAAGCCCCCAGGGATGTAATTACGTCCCTCCCCCGCTAGGGGGCAGCAGCGAGCCGCCCGGGGCTCCGCTCCGGTCCGGCGCTCCCCCCGCATCCCCGAGCCGGCAGCGTGCGGGGACAGCCCGGGCACGGGGAAGGTGGCACGGGATCGCTTTCCTCTGAACGCTTCTCGCTGCTCTTTGAGCCTGCAGACACCTGGGGGGATACGGGGAAAAGGCCTCCAAGGCCTACTAGTATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGATTCTAGAGCTAGCGAATTCgccaccatgaggactctgaacacctctgccatggacgggactgggctggtggtggagagggacttctctgttcgtatcctcactgcctgtttcctgtcgctgctcatcctgtccacgctcctggggaacacgctggtctgtgctgccgttatcaggttccgacacctgcggtccaaggtgaccaacttctttgtcatctccttggctgtgtcagatctcttggtggccgtcctggtcatgccctggaaggcagtggctgagattgctggcttctggccctttgggtccttctgtaacatctgggtggcctttgacatcatgtgctccactgcatccatcctcaacctctgtgtgatcagcgtggacaggtattgggctatctccagccctttccggtatgagagaaagatgacccccaaggcagccttcatcctgatcagtgtggcatggaccttgtctgtactcatctccttcatcccagtgcagctcagctggcacaaggcaaaacccacaagcccctctgatggaaatgccacttccctggctgagaccatagacaactgtgactccagcctcagcaggacatatgccatctcatcctctgtaataagcttttacatccctgtggccatcatgattgtcacctacaccaggatctacaggattgctcagaaacaaatacggcgcattgcggccttggagagggcagcagtccacgccaagaattgccagaccaccacaggtaatggaaagcctgtcgaatgttctcaaccggaaagttcttttaagatgtccttcaaaagagaaactaaagtcctgaagactctgtcggtgatcatgggtgtgtttgtgtgctgttggctacctttcttcatcttgaactgcattttgcccttctgtgggtctggggagacgcagcccttctgcattgattccaacacctttgacgtgtttgtgtggtttgggtgggctaattcatccttgaaccccatcatttatgcctttaatgctgattttcggaaggcattttcaaccctcttaggatgctacagactttgccctgcgacgaataatgccatagagacggtgagtatcaataacaatggggccgcgatgttttccagccatcatgagccacgaggctccatctccaaggagtgcaatctggtttacctgatcccacatgctgtgggctcctctgaggacctgaaaaaggaggaggcagctggcatcgccagacccttggagaagctgtccccagccctatcGgtcatattggactatgacactgacgtctctctggagaagatccaacccatcacacaaaacggtcagcacccaacctgaGCGGCCGCAAGGATCTGCGATCGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACGGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGCTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGAGGCCGCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCCTGTGGTGCCTCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAGGTCGAGACCGGGCCTTTGTCCGGCGCTCCCTTGGAGCCTACCTAGACTCAGCCGGCTCTCCACGCTTTGCCTGACCCTGCTTGCTCAACTCTACGTCTTTGTTTCGTTTTCTGTTCTGCGCCGTTACAGATCCAAGCTGTGACCGGCGCCTACGCTAGACGCCACCATGGAGAGCGACGAGAGCGGCCTGCCCGCCATGGAGATCGAGTGCCGCATCACCGGCACCCTGAACGGCGTGGAGTTCGAGCTGGTGGGCGGCGGAGAGGGCACCCCCAAGCAGGGCCGCATGACCAACAAGATGAAGAGCACCAAAGGCGCCCTGACCTTCAGCCCCTACCTGCTGAGCCACGTGATGGGCTACGGCTTCTACCACTTCGGCACCTACCCCAGCGGCTACGAGAACCCCTTCCTGCACGCCATCAACAACGGCGGCTACACCAACACCCGCATCGAGAAGTACGAGGACGGCGGCGTGCTGCACGTGAGCTTCAGCTACCGCTACGAGGCCGGCCGCGTGATCGGCGACTTCAAGGTGGTGGGCACCGGCTTCCCCGAGGACAGCGTGATCTTCACCGACAAGATCATCCGCAGCAACGCCACCGTGGAGCACCTGCACCCCATGGGCGATAACGTGCTGGTGGGCAGCTTCGCCCGCACCTTCAGCCTGCGCGACGGCGGCTACTACAGCTTCGTGGTGGACAGCCACATGCACTTCAAGAGCGCCATCCACCCCAGCATCCTGCAGAACGGGGGCCCCATGTTCGCCTTCCGCCGCGTGGAGGAGCTGCACAGCAACACCGAGCTGGGCATCGTGGAGTACCAGCACGCCTTCAAGACCCCCATCGCCTTCGCCAGATCCCGCGCTCAGTCGTCCAATTCTGCCGTGGACGGCACCGCCGGACCCGGCTCCACCGGATCTCGCGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCAGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGAATTGACTCAAATGATGTCAATTAGTCTATCAGAAGCTATCTGGTCTCCCTTCCGGGGGACAAGACATCCCTGTTTAATATTTAAACAGCAGTGTTCCCAAACTGGGTTCTTATATCCCTTGCTCTGGTCAACCAGGTTGCAGGGTTTCCTGTCCTCACAGGAACGAAGTCCCTAAAGAAACAGTGGCAGCCAGGTTTAGCCCCGGAATTGACTGGATTCCTTTTTTAGGGCCCATTGGTATGGCTTTTTCCCCGTATCCCCCCAGGTGTCTGCAGGCTCAAAGAGCAGCGAGAAGCGTTCAGAGGAAAGCGATCCCGTGCCACCTTCCCCGTGCCCGGGCTGTCCCCGCACGCTGCCGGCTCGGGGATGCGGGGGGAGCGCCGGACCGGAGCGGAGCCCCGGGCGGCTCGCTGCTGCCCCCTAGCGGGGGAGGGACGTAATTACATCCCTGGGGGCTTTGGGGGGGGGCTGTCCCTGATATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATGCGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATCAGTGACACTTACCGCATTGACAAGCACGCCTCACGGGAGCTCCAAGCGGCGACTGAGATGTCCTAAATGCACAGCGACGGATTCGCGCTATTTAGAAAGAGAGAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATCTTTCTAGGGTTAATCTAGCTGCATCAGGATCATATCGTCGGGTCTTTTTTCCGGCTCAGTCATCGCCCAAGCTGGCGCTATCTGGGCATCGGGGAGGAAGAAGCCCGTGCCTTTTCCCGCGAGGTTGAAGCGGCATGGAAAGAGTTTGCCGAGGATGACTGCTGCTGCATTGACGTTGAGCGAAAACGCACGTTTACCATGATGATTCGGGAAGGTGTGGCCATGCACGCCTTTAACGGTGAACTGTTCGTTCAGGCCACCTGGGATACCAGTTCGTCGCGGCTTTTCCGGACACAGTTCCGGATGGTCAGCCCGAAGCGCATCAGCAACCCGAACAATACCGGCGACAGCCGGAACTGCCGTGCCGGTGTGCAGATTAATGACAGCGGTGCGGCGCTGGGATATTACGTCAGCGAGGACGGGTATCCTGGCTGGATGCCGCAGAAATGGACATGGATACCCCGTGAGTTACCCGGCGGGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCAT  I   5   +      2   )   '           0            8   )   +   (   %   %      /   *   '                   $   !            2         '                     *   #               2   $   "   5   0   "   "   "   9         7   8   0   *   0   $   #            )   "            !      /   %   '   '   *   %      &   2   ?   &                                                             !         .   "   (   "         *                           $          %   +   *   $   *   !         %   "      $         $   !   .                  &   !            !   $         
         "            $   #      (   (   =   &      !   "   *            %   *   %   (   $   *      !         (   (   +   *      )   9   )            &             (   $   5   E       !   !   ?   '   &      "   &         7   '         3   7      (   4                     +   0      -      ,   4         B   (   !   %   -   5      /      5   $   '   @        5 ^ Àš›�‘¥Úë./08Õ9I
OPQV}³´
€^_1àáTUmnvwxyz{ùfèœØ + §ÁàhœZtÛìù19lÖøVãù:J
R‡µâ VZBƒ¹A9géæÁ‘0› h! $ _ ü €¶ÁÙì
C
‚
Wg~¯éè$ý28¹ÚWú�B!2 >RôüBmz’¦å¼Bé4~	†
e*œ�›ªõ¬ �·/—`÷Žû;o|�|.•ñÙ p!) ¨ï8ùW†ôy	K
A}€›ÔS¶³;Y€–îœŸÀPSOÇB!.3WCh’' œÂÜáií:;³Ìª	Ä	¹
ˆ6©¨JvÚ;„ã:ôŸç­í1Ê Q!i!y!  7 �uú2Â×K	T	Ð
$Æ€nÍKºàBdyêÌ¥|ÂÝœ é   .[ÜmÉßäú)	q¼a¾K×+E‘ã)[ÔSs�ð0 ‡uñ Sîñoœíý	
ƒ
ü
hu°dŒ¦©HHw:d3‡ž²³ˆ !6ûp¿ÕOvR C! ·F.I¤	�â—XºÏ	_yž´û(I‰ % ` ý Â¿¨ÉY}
MêDŒÒ>ƒ19kº-p×RžÓ  �±²ZEh¨ÚD
³ðX’é%@þÔ(Ûä¥¥žÉ%à8 ?SÊÓõýCn“ª·½CJi	x¨#{�“Ì>eh›42=aµã!<Oü	;�‘šm}³Ì2s’ï¼®z � Ú q!) Õ “Ðæô{®·bz¬³	½
ƒŠ+\oœ­¸0øc6‘7Dn U!+ Y§êŠ½Ê5Ý
‡
3fœÈöüA³7“hmœ½ü?p}çË`­@/ã–ËŽ( Ë Y{á@IŸÑð1	s	4�K«‹�‚]˜t—­ÀÙ$��±[	&Ø¢òý% …½XXÇ÷|	1o~�T­2™_‰¼�-4Q…ØX+IQkn²B“% ö Ö‡õÁNPáz	á	L
÷
œÕGp·–<EZ— VTPnCÌV‚„iÏ»k ù  úÿ
BH`�ÁiƒÕ@„nÈ#9±U � / 3 ©·ðè9œËh±* /´>G‡Âévï` ²äðRˆÈ(dÅ"/4D†dÞ	¶Q®öF!*  ÝâjAî§´ñ	º
ž×Ir ¿X7YªŽÊOÎ1…íä èÐí…þîý2óR!' N t Ï BÃØåë–n;RÍ^	«	Å	ã	w‰¹Ö[Û•½~<hnº;õvt²Ë Ý z! C í Ð¹‘	¶	ê
V©w”Vâm j!‚!  (e�ÍV	uÐ<-
–
c¹Öönw"{ùK·£ÿù;®³  Vž5>Dv£VÉÓ	Ñ
�¼ò¦·XL{áeA ! 8 ø r�Õ3Ã‡ð»Czë‹ÍƒŒ³�  ±­cØªÃ:	U	a
²
ÇÚ�o~)¦iÃÑÒ$ qû~” qL	 
VÚ\—ÇÖé%ÒpáÎ2}ˆ�ÞêS¨ºèê û !! ½ÑÞ€ ï9‰àN
p
ç
ù
É0Bb1	KŠ�,/\k^TNa£ K\Ý'_÷*ê	Ã¿ÑLäé#2Õ0t}~ñ*t! /nÎÊåûóô	Ú
½¿ž·Þ®|R¬O—‚ƒsN  ò d�rY€ãš	r”˜Û³ÔÕeØFïE’¥*¼M½} 2 ,Ñ	òÀTïò�»î'x/	A
{
„
c(v±b~�§ªìFIš¾N";Ð�a‹qíÓÖP« t cæ3~¡spñþ	ý
�Ôðt•ÅIÔ!¡‚¸ÿ@L S D! '§MŽv



s
y
• e´‰"¶î3~w r ' y ˆv¡ÖJÿŸ
DiÆebk
x4ˆŸ³çõ$7üE«5À©Ç¾ ! 
GË´¹)J€? ‡ �¸×˜Hy&ã	¥	€‚_°ê�ðÐ`zŸ;ö= ! G ¶R‰/J¾ã˜˜¨ÑYw»
µõüŠt  ƒ^²OŠ—[ÃkœW  þ e³Ô
LE…ñ~2.a,%l*\_Äìúƒ ö ! & � ˜ ¤ ¯ ‡R#?¨×»†ÀØ¼.J³Ô  * xÃý)©ÀÝ©Êü n˜
u
~
Në†÷ü+„ò:lBr¯q5•S[Q…<!# a óZº	É	ÚOƒŠ°g�ÓÓ¨¬ÂÛ©· DÊaBŸÑ ! ‚#³	ú	Y’ÎJ“öÏ&Và‚×Æ6B¦{‚ 8Ì�òz�©>	´ñ$êvº)åûžíåK¦jÊyï1 c “  , J²á[F†E
Ì|ñ:Aæÿ¤ÂÕ¶ÜiŸ&X H�³-i£Û†	Ö	ì	¤Au¤ù�ˆAR �[Ôpá2 5 òxØ¦ÇDfq	{I‘”?f5Fæ)8>\bŠ"f1=R
M4É49Ždn±¸Í½MÑx $ @TËÔéo”«š·å
@œ/+ˆÊßPi)f
O´t°Q¹¯{ Û r!" ‹ ¢ F1–Ô¾K%Ms|i“Ã˜®	TÎäÑùœ›u*3“ë‘ :!5 f öþ.7D]êì¸j	1y ©$Í-bïzÅ3y›¶*,L–$týð<’”!b~Ÿ­ðToøU¦ 0 ;S\”—Ñ$|¯e¸c¡ 	€	ƒ	Q
„,È§Þx1�25dºÇ©&kñ’•«cfqú  ° " Ö _¦!<´F]äáªõ ùSªKˆø8_®ÛWÉH!V!`!"  £Ã	{l	2
\
¾
‹g¯Qq¹{¡Qhv-˜Ç7YòçNSo! '!" h ’çõ­õ3p�Ä.p®ñ÷ W¥É »˜7dn“7E !9  Z�£P•ë!BL‹¾Ç©Þ
¯
`‡¡ý‰æm¢´ò”ý
DG~ÄùSV>a¬Kœ®/+0Q	F«ó  ø<„_Œ•³6
ˆ
Ô
,÷B~„8ÿÍvÿÂä5h ¹0ÿË!	Òáýx´An5�qÌ–.ˆÅò7—�D 7 ¸ G¨­ºj$3¤Y	4g��«Éåñiô#¾@FèÇ#€�¡ÁAd£¯ý$,¦ÄÌ~™Ø† À 8 Ì ŠÇ+9žÍO Ñï&5ATyÅ
d¬Œ�•™¨«´5Lk€˜¸
%.|&Fhà'Âêó-þ]!0 { QŒ½âŒ¿ÚÒ	2	Œ	–	ñ
",�ò83Të2æPdå�ÀÇÜÚgéŽ²G\
‹˜æFb6 ¨ * |î AJ‚ñfút	¯	¦
k¢W^gž¶×&L)ŽÍß|®:µ;—õþØVÙo0 Z«`m¡áY1®Ò@Á9
T
à
« :")PÏÜƒ^quÁÊë6NŠ®q�÷6Jóé*FI£Œ $ # ] û =Q¦ßûAl™ÀYs�ø}	U®	š©.–öü7�Y¸çÀ”ðg!o!# ÛîÈ	J	S	Ï
@p|`½ž5RÆ�IÙÌJRó€¤ï{Üè P! †¾ðYXn-£	t€‘¥†0j²,‡o³ Ê X©?HI¶a²¼
2‚3ËŠ¬ ¼°lË%?‘»Cm­T! ¶g°öO{	à	ö
.o=F˜Á_3QÄ×�ƒ…?jm­Ç"8) M ì '×äUm¦Q]	�	â	�ÖHq¸õ!WzUøÉZÍ”¶}“máøìs„üòl ² " ÷ =CbqŒÔ}©“ÂUÒ	Ù[»Õèo¥ÙïW(Šh‡Ðé‚Ñ@ ú  ñ &ˆâé™	ó	M
Ù
ø
“—¾/ÔDè«}¼ x årºužC'ŒÅa` íÒª?¨ÿ q ½  GQ]~± ÊZÂj³ô:>õV ! w²ÜûÈ	K…‚„"}§ÖAÁÚ…É$ -kAIP²ù‚  ! ËŽà…	ë	@ÍIõ9ÁQäì5iob / 4 Š èñé0™ÓÆ¶$0?HÂaåS[‰)eÉÐÞ#0QLh3eï3N·ê¯LP÷Ð9!%  ‘:–dÿ‚	ô¦Ãàðp©ÈŸJPgÆ6‡÷æR”ªbeÚ ¯ &!G!' · ¸�^i²£¨ 	† üŒˆw}@¡³ñ4ÌEÃø«
JÀ¢ü*+4˜×' ªÆ_l�Ìà?eÀù®	S
!+ªµÄÛê(OpÉ­fp5èßôH—åé* <¥ÚÞík˜¨ï>Gmµ`±»
?{
sÊ¤«Åû�†Ê$îz¿Ûï¬ç S!f!n!% ë &ãBp‹¨T�	Ñ	ß	ò	ØºÀÔ^Ó÷ØçYÃ“î€’>÷‰|!ƒ†?  w v±ÛFßÇ	ê	J‹Ä„Y8P¦²ãìhÑÿþ=ôøa ¼ &  ‰ �Åçkc¿	­	Ÿ‹¥oZ¬IfËÏÝ5K÷2îåó¡é)a3Ï2 v ê %;�¤ÄÙæì—oŠEj=S¬	Æ	Þ	é	zŠŠÉ×HeXÂÜâí=ëòÉö#ˆ yÚÎ» e!? O P u › Ø é CDÙš%&'()*/07M<¶Ý	xyëÍ–áìi!"Z[uvwx³ØÙøùËÌÍº Ì Þ J!K!X!b!c!d!& Q Y ‚ œ § ² Ð Ù ä —#›þ8>Îc	ºÎDXa³—¾Ú`o»\óúY!{! U EÚ+1N·_	ä	ì‚Œý\j<w:>´àÍ ß L! D 4$¹õ ?Ï§	·	¬
î
;•´a•-pSû4°Z!|!  R Z � Ç î Ñøû	d	ë
EMbö˜£¿ÅŠÒÝMn –   k!ƒ! ƒ � ¨ œËÿ9{º’	‹
_ýÏYª½xaÊ¡¼Íô&%  ³ Ñ Ú å r˜u�HW»Ûó¾ŸWã]; )5ÎºÛ
íd×æx;ã|Lk�_î¯Y¥Î   V W,J(Ñ=å`	g
	÷·8J¬A¢þ]�¸Â ×ú¡;M! a2O+víå	.
ƒoéúë&�¤bÝµ †
´ à ! Ã Ffžgv¸À	—
Æ
ßûº#U¿,ú=x<t?á] 
! W6jžºö@Ý}Ê¨	
9Wóþ}§<š9âqw£Œ   1 ?VbÅLW8	(Á±½c£µ»ÓMy|.TÍü±¸ E Ÿ%¥w!ý¸	Ô	�ˆï–¢¸Yý·Â!}! 5E”W¤¬Ð		¤
­
Ò
ï
^UD÷b–š‘f54 B v [! [ È ï ù ÒÄ¤çô
N©=úiNo ­  S â sŽùüCa	ì
Â9FcŽ¡¤ÀÈñéì†„Þ# ž l!  = ‘ y¸Öå_	·À÷ˆ™›�ŸÆ‹ÒD 9 ó4e	¼{ÚÓŒÎ�´— ¡ „!  ) „ {�ì6Ä1;	V	n	“	^
¹`Û/«y”/[½y'eÇ   "‡®dÈØâ"|T‹˜È¾bË*FP§jÒõ#!  ? I l “ © ¼ ²ÄÌç:Ù4
 
³
À
ÐZjùþy�¢÷Ä\Ó ž MT÷ «»b
Œ
þåx‚pÎ”& f  Ò ™r®ºü’�°7]S˜»ÜáqÎÔð
X^fˆqé.   ´ æ Njsv‘¶Uú!r5	
�
%äi5×¼S’ŸÆ«ôÉÏô ½äUÕ<×©	 Ä " ƒ‚•è9�	M	(
9IÈ&šÐŠÜ°¿þ~‘ßKTžë  !)!( Û Å�
(l™	)	�
WÛ;ÚgsêXÓìªâg[ÃÛ[3‰ëe»9 ü " ¾ßé÷6ÓQág	ú
îeçyú<c2}
u0@lžUÕáš�™ w! ÏÜè
/C]‡‹-bMÜO9�°Š¦ *6aÏÒû�…‡ðq
ÊØïîrLŽ€]®è_pIºZÏ * Š»NZ¡:Š“1@Š	O
?Ð*10äËžÃJý
9§l•`ïbm¤Â| ?.]Þ+Q	¡	×
>úÀ>M§£ÿG^fØ1ß~¢Åò+kN! W 	-K)¯½ëa	
é¸9ÒmËBV_å‘Ã3ËÕX< ; j ñ`�´æø	'	\5SBßêe¹uØû¶«  %LXë(Ò>`E	h
ÄçøEK­Ê$ÖÓçu! bæî/
Æ„™pßs}SP¥'”Ž› . O ! ^”õNæ	¾;¸ì­ƒcÞé‡ íPowüwÕÛ
ŸY'&„!µ 0!   03Ï,ËŒôõ	ÀêûŽ¯˜Å¶t Y á !†!$ ó GwÁ	»™ l�°Îé¯!fÌÙí½Ýsy“+*ÉNC¶È~ £ ð  Ä g"›ÂsäG	›	ž	6´ÆGÅGðó-v¾Ž@1›â^ 6!  ~ *ežÔb�A	
j
Rsü.ºÕâó"FVšÀìµ>ÐåG% mŸ'hçZ¹˜
Ç
•à÷ÜrŸ$MùkÖ„¢»¦û}‰y½=u¾!!+ ~gµ-k»÷AUè8Øë3
B
H
�
…
d)N†²Žô«ÿ¶#lu¸@ø{-A!* -
7Á…Þó²(~Ëx	©	š»Ó²:XíJ›¿OD¨AÅ§:crxž¬É $ ŸíðD›§|
Lc‹¨GÑ�á=‚Ó'–ã¤�Ôú'HQÈ$uˆ Q * XÒ’óz­‰ž¼ÉÐïy«Ü0	
‰w›<~–´Øb›ûŒ_™1rî�×¡�ü ! 2  çWÆÀMàþ
)nÿ1Gèu‡ÔUcƒ mÎAT j ø E!  B d@ÏtÏð	,
•
vbÕÿmv¾¸Õ¼0¢¹UÏý�! 4¢pÈ9	ÿ	`
±
UŽñ–Æ¶†z}1�²¹!% ¼ŸcÍî8œqöXòo
ÂÈÚ²ÓJd¤Ý­{"1N]/–M¢)rM "  &}¦I�þwð.		


@
x
z
ba!Ó€Š#µ·þ24ÆK  F † 	ÖµxNâ‰	–—ï¸s !$ — ® d(¨ÿ—¹	

t
ö‰fð*ñ§®¿¶Z)^Ã õ ~! + ",~�…=	²	Õ	ù	£‘£‡£¹@µÿ€ZAÓWxî0  6\w•×¥ep	ä
r,Eyø›8 tž°¬STw $ ¥ R# 1
[
EÇ-oöVw¤ô 1u4¹c—ÆX’%M6^­È  !! ¢F#KÆ X	
®
Ó
_àäåló"FRU.Á6ªgC … ¿ . z ‰XÙN­Ñ
	•	8
¥
ß
ð
jîSfÖOc5‰´èöIEgý)FÁn,a5 ‹ \! \ É ú úŽHÎ
Œ·>º L ð µalÓ¥¯èõ
n½.µ|ëè�7 P�qÌ|Àj@Op  ÅµOoPÂªû©*KÖ®  ˆ °Úb	Ð	Ãƒ¥±{¾<÷> m!& T � š ¦ ± ã $Ž–£Ø‰™ý$Dè	�‰GdüWÙàë_êñ ‡ò÷ß¹ ! � q3t�Ê÷úz	¦	Š
«
í
:õ`¢Éò½,žRÑ:%L$ Ÿ  Â ¹fI'ä@ê%�Áœía Ü…	! UViK	£
Àbî™Ò¶‹ u  á ·äB^`¿Á8�šœž Ç…!   ( > H ’ !†zÃÇ×áæ!SŠ—0¸ä.øxŒExö["$  M­¹	T8¯™žÅÏ©‰ªÍ¯ÓZÂ¼ÚZe‡ýÔJÖ  `‰„~5†Pf	î)ù\†�aü	¦HÛÔàl™‰Ž˜  : >-êð_€³&	D	P	Ö
D=lÊAdÄæjµ
 ôMv‹˜:- X /!…! š
Q-¹á€Äº)u½|ˆ�ÈÏ0šµ¢ ï  }f´y¬ˆš×2w	
€
™ºM±•t˜zù&Ç#‡ûÈ "  A ÿ »žÎÌí7›õ+
_
n
”
aF†Ü¬z0/\blÎ0Ž÷ €! * … – ­ Õ|þ}„<	
w
¢õÒ)†¾Y(]Åí ÅW	o	”	7
Þ
íÕãò0!3Ds-]m+S`f„ Š  K ´ŒpÍ
¿n‹{¨Õ$ Ž ™ ¥ ° Á #2¯eˆÉÙ#Ï	ª
ˆé$ôûØß¼›+Q½ñö9$K¸ #   ' à ¬ÂÆàã TUhS¢
-7�©ÌÁÙY†ÓÕ   ˆ,éï}™%	PÉû@c(¥tG‡Çk/™´, î .!( @ • ¬ | Í«Ëýš|Öô
6
m
v

Ý
ì¡ÔôÑ…s CrøX\�ÆR†(  J À ³+«ÁÅßèSo$	Î	¡
©
O‡ú,óúmŠz¤sšðÔ· -!=  	 ” ª « ½ ¾ ¿ yz{Äþÿ*ªÞRª™#	Í	5
´
Á
¥¦Þßýùklmn‡ˆ‰Ÿ ÿ£ßàá"Î67TUVW”ÅïF ,!& 
 m Å;qÚD!
µ
Â
§àøþ[o;¡Ž³â#Ï°08–Ûô•]G 2!=! Ÿ N�¼¶
áù„C‹â±hq€©Ãªã!^CÝÕ !!  Uôøi–Ú	%
�
Ã
Øz¡Ë%[Ðp\pÔ�­Eâb‘•#!3!" <?¬þyÍß»	Ê	"
=
X
ÛPÿÿÿ=^æy´ÜõË–' H g Ò >!*  b n 	ÆrÛ[E×c
¨)¶‘ÿŽÔ=Ü<ƒ¢ïã$ÐÜ±Ï1„Š9—¸  Ó šs¯û	·
±Z	™rákØ�7Y£«äÞÖ  ƒI»´	”^…TÐâWƒ_! ñ$l“úD¼÷ÏÕªÄC§©g_|r€ƒê!%   O‚ý“€½Ž8ÏâKŒÝã)¦²'iéBrñÇŽš"‰D* ! µ OV{V‘"s¦ý[	ò%�ÂÙ{&ó ÇÕÊê®æõL¾åkF¨=NØ–ð$!%  ç 9t5·ó¾	Û	Ä
ç6¢\Ñ½“±»*À�ŸÇf�Ö	ª[ ” å ( / º õw’�&€ªûjŽ—	6	
&
Ž
ž
&µØ� õüÐî¡§øËc’ 2 d Â Å ˆ!$ kÍùÑ?	åjÌëqwT]qE¬æ>É%’ÃVÿ¸ãÆz
 4!* ³Í×GÎàé‡Æ	÷	>
F
Y
JlÑQ  B Ã¥Ñ%·ÿ 'UŸI h ?! K@„\­N	}ì >_zÃ‹µÀ—Y !  =z˜Ë	#
)
Ë
ÜòÉ;ýç›Ý±Ý‚jÌ.’à* ˆ Ó ì !!*! - âÿƒ–:‘	¼	:ÍÏË'çÖ±ÝöL( ã  c ª¯´.\FŸv¥Û’ÔIÝäÑÝÄ…C¹Õ\4›å: ( 
2DÜä�¡.j<ö‡	d
6<Ü<Íhú‰HÐ2Ü:&qfÎý (  s)5…¤Üm“	×	í	‘
ij*t ±Õ>ö=B„\\²‹³ŠìÚâ+ o Ü IÇ‚†¼ÆšØ*	X¥B©Z­·ëY‘�YØí	S£«ãðh‚%˜¥ßv¡¼* ¿óy¤Ùäêø7Ôâf|è›~ç]0ßú:SAÙ‚V�8eå›�/š ×  6 ›Ã	¸
•#Ö*Nu(-2mÓßâËìx!) t°±§ÈERgHü	û
²ïCGv9?c‹±Ÿ 5o¤¹ÎÖ^x¾N�Òß9 Ô Éà¶Ïh	r	[§Ç"Jnz’û
=@dgŽš²36\1s’¿â#gl>Næ5Ê:ŒZ¬²â6Êy � ) õ „Õ¼›Ê0F¬•D_†U^ˆ»±,hãïm‚'c„Ë*HPÝµP±ºõ°€  s Î AÌê•¸µ	é
X�Ngìþu:±Ü  U°p¬ÑàR‹§ç! ÐÝJ^æ
A�Ð0‰Œ¶.î~Qj`|»L‚| s!& +bÐ2Õ¿L&r
”Ó}ïjs”Äë	E™½MÏæô�Dªì}s! Œ‚—ˆ%½›§^¯Ðéœv<   Œ £ Óòü†mQÙNû¯Ò`q«¨+4”C»`[„ëÐ ;! 7IG€ñy¢Ë#t{ðø�Î
Ußåu�ú�ÖÅJhz�’ ( e ¡ -EëzL’.—î*‡­Ä(7xšÍ+K•	s(È“ac)n¸¥ $ g ^[¢Â;k	P
2ãf®PÝŸzœºR—©,¨mñjðpùV  _!5 ÷ÿ/;ƒO‹¬¹þ”” 2AªÑ+ƒðþ	Cuþ=•Æ"-‡�› c®Äñ#PE¥Ã}ò E *8P‹¼í 0�‹¾‹	�9¡Ðñ!%(7@V]x	%2SÎ1åc�”ÌÞä{€§ª³¿ÆÛ4Kj·ê	-9M{%:ò–E×UŠE§   " P@W¿rW,	I	R	¢	�¨•õ/6Hi±»Ë¯æò�£lO!! <T%f|’\	˜	Ø
’-ôyç2¤ÈÖª'làl¬ÆgrÐûñ± ! Êä\}°¹t�„	&?Éô!_ëÀÙ4ó©,>§H ÿ ? ¶ Ž/]^•˜Òß#>d¢§øþ�	R
 …©óûÃÚ|Áß'?No¨Çž3 eÈgçö2MI¿G“–dOÙäè— %!8!' u§FÞl_°¾Ž	>	¤¹£Ó]ÒnöÄú«æ’4J‘ög(`‚«` æ & X × è :".6Li
=µb	Ü	êÈÌCW`‹²ÁÈY9=×ÊI!W!a!  Æ 
¸ôG^üL”¼Ä‰ Ì
3¯Ü• " 4`*uì¿	f
Å
Þúåè¶7:Iâ«¡T¾+ùÖ^s	X¤\ !& 0 “¤a�ÄÜ«ü|7	
']8V°ýT‡C|¢¡ºö™8x�v¢üÌ·Á 3  < xò£æ	ó
¶Ñ¨Ùè<ùh" ¬   k » LSöë5m	3
]
Ÿ
¿
iw“ý.ZOd“e Æ 7 iÄ‚‘µŽ'�çù
k�˜		(	4	'
�
œ
$8Œã6:h4RÙfrºÛàR‘ëfÈï	óTd�p8 Ã (!' ÎúMYèöÒ’0?‰	.>Ïí/qÊtÂI­ç8?o” 8€¹Á{v! i $®¼ 	[æè4=ùR¦ÉÞU^Fe×ÊÒÔÞWª  ]“ìÔÅXr%&“Ä�šè  !3 } l!&)æÁÓa@	F	�	i
ö5kqžÅFL¯Íèòø!j® ƒ¡Ëì¼Üòrx™ë´BäFÇ!5!7 ‘,„çì7C¦±ÈÏêªÛG
KˆÒ…šŠµC•³×@œÄà¦Ò&~kú·?^b÷0�,�œG P @! Î¿oÇßï	°
TmuÇþþ0lÙuÒç·…T•L
¡(qi !( c´–+HŒáˆï	-	±	ø	
?
a¢`ð?þ¦­´@µÒ1V/ J ô 4 [¤Q"Ø"Md0
Z
ã
RqßNä,nõ+v£ót¸EHÅQTW(L�¯ÀR ¬  ù`k�O®´m~¼-{O¶Áêç6 € p•¢öç	‰
?V^ Û  L…A–]7®¿aí ÄÎŒw® „dIZ! _=Lu…C	O	
Õ
,¸í(àøC…9<¹`Ãßå˜ˆi ºx‡™ƒv	*
“
˜°â( —kÍ/"‰  ß 1îgÌ
è6œÞ'ÆX…8˜+ í +  Q p{ÕóC"	Ì	 
l
¨
Ü
¤Ý Óóø†žr²Þ!™Í/ÚóŒ“îE ¶ +!1!0  €>ýxÌÞh•ÖÙ	$
<
W
×þ(y µÊ$BZ�Ïá<þ<]oÛîƒ‰âá�ÜÔ "! ðHk(~¥èjA�™¢¨!- . ¹ 4Ð%�¥ü	Z	½	ŽÁæò�°D¿=éÈ$‘ÂÆe÷þ€§·MÅ Z Á ä ‡!  ~ÌÖ —Å	ö	Ê
kÎÐÊëüÂ$�°-) ‡ â , 1…©®»Cã- 4„;’õ5;hžYi¬Ì°HXõ×GB¤²Þ%uÍšÙä4 È£°µÎãGÂ	Bš¦Æ!m�±"[ÕM‘¾/°Þtù',9åÒÞ‹áÊ]w5Éë.œŽ  r Í ô ¯É]E«ºÏ°gµîl�í&}ŒGëý{ºKôæ  H‹xP¡"¼ÒzêšÍÞt¦åó€By; B d ),:ŠŸ«Á/ ð$'6U\wRe­O‚‘Ü“–í†©¬¾Ú6w™¹Ì8JQz”¨+'iÅ,†(DÃÖOD·ÿ¤ ñ ^!( �¾Éãq[=œ÷H	—	‘¨ónÆæ”ô.h°¿3H�¦ÏãçGþ 7!! Å 3hEÝ	kt^�	e
=Ýù£µÇHûÃŠIõ]'r2£_ !% hKRœê4�¢ÃÛå	3	›
ò
#7â97U3üe†Ú’w�;gœcÀ7 - | \ #å‘À/»Óˆ	œ	Ÿ	-Z<=Î4ìçQis‚ÁÈÝHÛñê³ÑÝŒ™AÀ© !5 !+ƒ*6c¥°nÚ,	°	î	â
Stý/_kØÞMtÑã+ô*u²Ö?›¶ï>j„¶]³ö?Kœ´¿Ñp  o¡jKtB	­l+·»'ß`UN]ÿÚæH/  Þ f†ÔÝòBg‚”u	Ø	
;
V
k
’
§
—£Ÿ¯Ö'#�Îà;\÷Ú…í ˜ÙáW‹íÛ }ï„BŸÕÄû	É
jXh}¤¯GWÁC<Œ§ã5 q ®(‰¢ÀÈâ!Dª#[QN¹ìZ…½v�Ëì7P‹§¯s¥äòø8&êüŠ+zAJvx� $ [JÈgpZé3]›ºš
Y§áø;¢ûm…Ùr“Â
‘¯ô¥¿¢¿!!' Ý n iƒ¯ÓAÃÙ+	:
U
È
á
–¬*®ÝsÐÞ&_£[ÙîTMiƒJ›¦¾à@ p ¬­abcdefno‡ˆ½¾¿Çè2[\Â™
¦C ¡Ý%¸úl„×Ø’¼OZr¤¬­®ñ7KL~GHI™š>?@w¤½¾  4  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        5                                                                                                                                                                                                                                                                                                                  >                                                                                                                                                   	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     	                                                 	      	                                                                               -                           0                                             
                                            2   
                                                                              !                                                                                              *            
                  
   
   R   .         	            
                     	            
                                       (                  (   B   2      '   5      $   (   4   &   ¡   ^      $         %   $   |                     5                                                                                                                                                >                                                                   	                                                                                                                                                                                                                 	                   	  	                             -         0               
                2 
                          !                                  *    
      
 
 R .   	    
       	    
             (      ( B 2  ' 5  $ ( 4 & ¡ ^  $   % $ |   ˆZ  Š  Š&  Šx  �  –Æ  žÞ/ÃRaì]™ô :‡ˆ‰ŠÇâãÀÛPÃB	u	:
;
t!"lª«+[Ÿ¡¢ì‚“–v¾ôÙÚ
…òóKLMN]ÃÚs^(¹–€ô	Š"Ë | •
MQA5¸ê
!è*î@›YÇh˜B	u	tl+…Ëé.0QoGSºØA¦¾Œ¾¤ƒ	¦
‡1È 8^É@g”Ä“ /8q«5b”¸Á%˜¡¾ýÿŽé’õ!u¸*fê/Ó‘’¸| =`Ñ	äCs½‡1œ/äCsäCs�ç˜(	
“p v!²²Ò(ù®ù @aË?YÇyËûÒÖ”‹ÒmÊ.Öá÷aò/û¤æûý�€¿oé±Ö(š-Ùp€��ƒ?ý	{‘å×…ÇÞ!p“i>>Ùpà 7?H¬ïÉ�ÔÑÝ\TbâóV ‡hd#oœÌ­2µ3‡²ûD«Å3 ð Gäˆd/¶×YÎ àž0	Ž~–Ø#ŒZüœ § þ8¹ÎXï	þ
×y$"!íqï‰É#0Ì�2µã� q
ûNp
KhÿÂïD5¾ !5!¢ÈâDª¹ø8�ŽÈ*P#³å›¬�4ˆŽõ5!0	ØŒ©¦he�ÈŽ4D.¨!#Ì2µñ	:˜¨Y7 2ºyÌpÿ	æ„ÈZ›¥=Q"O	Õ
<v¸Ãiõý·¨#Ìa2µ#ü;}ï“` ‡œ¨§YÙ3fhœd³#oœÌ­€�‚2ŒµÇÒ3‡²Ž3û@D¢Ëž„Ò@;õÍke€ä pçÔá–C»`†íq­ç¾pq¢£â+EœÛ6k†¯^Ùu	=—«¯7Uºêüs“”6Ãô/Þr¯ø78ijG¶ö›”v¯FÁ	!Ô+ÑÝÉ\›®m	dÇÍAÅ lŒ°½Èê*’~�QhŽ#§m´>+ŸÆ$,nõ£pŽ#¤Lç| ‚›Ëå‡L
÷
œÛ7Uü)©¦åhÿe›Â¬ï�È4ˆŽõ4D5¾ !/ 6	&ËûEœÛk†^u	=—¯7UüÃ ~ü�¿Çò xŒ�êšÍÔ ³Ç©‡"ÅáLP.iŒ)Éu”Ê*6ªº@7˜ù“Ž’*G´
/_æœÛ7Uêüs6#£\SB˜û†“K	Ùè$|‡Ýéé ú T‘Œ6Ûï¸ r& ¢âø86‡úð· “.Ÿv’CÕhD‡„ï	Òþ@
;õ×y$Í"!•þ�	 “däEQT¹–	#)	,cMs-'†	N€•ÔI#)r¡5!/ Í­m6	í	)
&Ñ©j”T\Ñ)ÔBÃ¥�Ê�ÑÝ7äjÌ.�ËÎ^2\d
Ü¹qÇÍÅŒŽ§…û62IODñ k€%FójQ"v¸Ž§…ûIek€ä  � öŠ\Ðò e} ö!¯±£Þë
�
A!]~±‰–jV Â 
= ­úK	$Í|Ýé VÚéÒá2ˆêºû ¾q+ƒ6¥°ÚH	”²Öô?›.h°j¶]ö�œ, áÌæÕ‡áL
÷
–C»Z£P\”—Ñ!Lc¡€	Q
„mÞ¢ò�2dÇ
DGÄSVKœ®Q’•c« Yz²hÑÚqœª�‚]—×EtÀ‘Ž�)¢ñà¢£âãšZ�/¯Þsøù89Šv.�  oý	ü
H R C!k?	jÌëqwãÆ4!²hÚq×E‘)à\”—Ñc¡€	Q
„Þ�2dÇ’•c Z£P!Lm¢ò
DGÄSVKœ®Q«h�	3	›
#7â93eÚœ7 V5Éò¦áßg	yú<c2
0áŠO Ñy
™
+9Z£P\”—žÑ!Lc¡q	€	Q
„ï&5TId‘¬?fm�Þ5•¢¨«ò5˜�¸"f2dÇ
=DGMÄ4SVÉ9hKœ®±'ÂQ’•c«þ x ]!¾q¢£âã+ƒ6¥°ÚH	šZ”�²Öô/?›¯Þ.hs°øù89j¶]Šö�œv.� Ã	‹gQq¹QÇòSo'!D£Ñ
eA æ„™ß}Ž›O Éã[œ¿3¦Gþ �uÐ
KàdÄŽ'
k˜	(	�
:Ùfrëfd8 ¥üZ	ŽÁòé§M¿ßmþ0çTi ZÛlãùâZH�¤A�R �GbQwîú
î>»éuÌÝs^ž¥ØÕò+. ¿¥ßüZ	Ì
mþ0ŽÁçòTé§Mi ä¹t�&_©>§ År&“�š  9‰N
0a£Fs	Täùœ‘ (‰À#[QNì…½vË7P§&+òxØ{æ\RŽd/ÎªÊóô	a
H¿V®�o~V—ânsÐÝæ
A0‰ŒÎ
.Uß~�jÖÅ`z�š{Í\‘Æ[	·
lÇÕk¹ 'åðh Ýg”Ø	;
V
Ö'#�Îà;\ÚíáÛÝjî´º
rªŽ…íîR!¸Ì~H&pãñ>	Iêv?å!†`Ÿ.y³L !âA§ñ	×¿… =#
É;ý‚àÓ !!Hn/ÎÊóô	¿®—sÌ~pñ>	Iêvå!yL ªa
V�o~Vâ+9žq	ï&5TId‘¬?f�5•¨«5˜¸"f=M4É9h±'Âþx ]!ä¹t�Å&r_&“�š©>§  ?½»Tüôi–%
�
b‘lw!: e ¡ -EëzL’.—î*‡­Ä(7xšÍ+K•	s(È“ac)n¸¥ d ),:ŠŸ«Á/ ð$'6U\wRe­O‚‘Ü“–í†©¬¾Ú6w™¹Ì8JQz”¨+'iÅ,†(DÃÖOD·ÿ¤ ñ ^!5 òxØ¦ÇDfq	{I‘”?f5Fæ)8>\bŠ"f1=R
M4É49Ždn±¸Í½MÑx ƒ^²OŠ—[ÃkœW · ¸�^i²£¨ 	† üŒˆw}@¡³ñ4ÌEÃø«
JÀ¢ü*+4˜×f öþ.7D]êì¸j	1y ©$Í-bïzÅ3y›¶*,L–$týð<’”!b~Ÿ­ðToøU¦ ù`k�O®´m~¼-{O¶Áêç6qû~” qL	 
VÚ\—ÇÖé%ÒpáÎ2}ˆ�ÞêS¨ºèê û !Ë Y{á@IŸÑð1	s	4�K«‹�‚]˜t—­ÀÙ$��±[	&Ø¢òýÈ£°µÎãGÂ	Bš¦Æ!m�±"[ÕM‘¾/°Þtù',9åÒÞ‹áÊ]w5Éë.œŽ 0 “¤a�ÄÜ«ü|7	
']8V°ýT‡C|¢¡ºö™8x�v¢üÌ·Á 3 · Ë Yª¸ÆY{�á^_l�§Ìà@Ii²êŠŸ½ÊÑð5?e£¨ÀÝù 	1	s	®	
S
‡
!+3f† ªœü4Œ�µÄÈKˆ«ÛêöüAw}‹�³(7@O‚¡³]p˜ñt“—­ÀÉÙ$hm­4Ìfp�œ½ü5?p}EÃçø�±Ëè`«ßô
J[­À	&@¢ü*/H—Øã+–¢Ëåéò4Ž˜×ý0 < á x“¤·äòBUVai�^`£ÄÜ«üK|æ		7	
£
ó
']¶À¿Á8V°ýT‡Cb|¢î8¡™�šœž ºÇÒö™Ñ8x�v¢¨Ùèü<…ù¶Ìh‹·Á  " 3 u ¬ ‰!*6aÏÒû�…‡ðq
ÊØïîrLŽ€]®è_pIºZÏ ¥ R# 1
[
EÇ-oöVw¤ô 1u4¹c—ÆX’%M6^­È  !ƒ � ¨ œËÿ9{º’	‹
_ýÏYª½xaÊ¡¼Íô&% }ï„BŸÕÄû	É
jXh}¤¯GWÁC<Œ§ã…½XXÇ÷|	1o~�T­2™_‰¼�-4Q…ØX+IQkn²B“Ž ™ ¥ ° Á #2¯eˆÉÙ#Ï	ª
ˆé$ôûØß¼›+Q½ñö9$K¸ ~ · *6a†¸Ï�Òû^�…¾ð*e‡ðYiž²ÔXbn-�£¨ 	A	£	
j
q
Rs† ÊütØü.€Œ‘ºïˆÕâîrw}¥ó"@¡³LŽñFV€†šÀ4Ì0j²ì]®èEÃø,_p‡µI«
>JÀo¢Ðü*³+ºåG4Z˜×Ï ƒI»´	”^…TÐâWƒ_!  7¶     }  !Œ        !Œ{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷ØAî~ãØ€à‚@zî+&ûxø«âÎ;o‹Üï€s‚â8ò›B¼wãÔ!õë¨¶ï â-º<¾cø4ƒ„hàëƒú€¾¸—n¨vØŽ÷�½¾×î"L�Ðš›½?«ÂaQ‡ÿPÓëo†Ò Ø¢â{núÛô�;NÔ5_ÝOû†cÞ¿ƒ„}‘×ˆ{Óí4˜öí¾âYN©ÛøƒÛa¢ß�ô�ŽÑ]YÃ6Œ>�4Ò÷=E£Ñ÷Ý	`Tø±b»_Jê”búÕDW•/…âÂ`"*"8 çêën…ãj�ëˆ€Í?®6†½wêŠˆ‚  9ko¡ÛjcêXáªÁCjµþé9Ñê?Œqe�dGIµ—XÌb«ÓÛa=Ä;Û½A#[‚§á¿v8€ï¸AøUA4àÛ=èN/ôþm-š˜w‡.îQ½mÝWMý~5ã;Â?- 4LÝ³÷KüE( h\ùBÀ%xHbÈžóê¯Ä EòfR4’Ï!rº«5À¨óÑwNëÍ»¾·3óÏçá=½Ï
Ì®<ûóâûáxËÏ.BR¥NÐ±ê/ïèçä{Oïs¨øŽŽ×¹Ë¨«*¸®3ÊtoSˆ|3ç9÷Ø1ÄL>?=�<Û|7ø\2¬N0DÿÔÐ<Ø}uQ7Õþ­?çã„÷!€ç¹÷¬7çý0çëãsÛ]pÃ�õßÍ±œAÃŽ7!:ÓŸ9Ä<<Èl1ëP‚|:ÿtÈn·ÇQ¶óMsß Møßör8 4ë?7ëOŒÇ9çN²=ØÞµ=½ÿM¾4\ ÿb³ëÏ2�Ó]ñ8²ŸMÏ­GÿlúÏFüÎÒËÖ¸òóÁü;ßìÄRë—croÃù‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�…m~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰´¥è©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.¡íXbÖ’æÑNn¿¯ Goâ¥š•ÓH¯Õà+¦oèî_B�¯‹íÐ\RþTWH4ô*OðZ¾ª�?h&ªÿ&ÿô"ãCµDÓL­TÆü�~¿Èü/ós�ÀÀ3ÁðLü;8ãÿ“ –�¿€  ‹øfæ]EY™™e)·%$]…eÖe¦É„¥n•KrUmŒ¶¨{s)’µ[Hw§Í¡ÔÜûp½L1á¾V7Lpk3š‘Q5šµ62Ö©Ín®@¥¹8ÛA¶l°.ª¼;KlýNæà6ÀQOÈú¯ÌB…0Ø£Äxã�¡Êsr§Øa¬ƒØÜú—^++Cß€gÒ²Ì:¯t¯OÅ3m5ü]gÃ<�#Â#F–0Œ3€fœ´Ä	¾<ÑX�ŸòŸÃ3 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶  ¿    v      a®ZTR

BASE      KF  xÁA  ƒ°YëñÀ þµ,9–élkÎIXÍs!¤s†d•ƒ`ÍÍÍI·B,§#rc	K‡:RIÓtNEdab$d’Ä‚†©nI#“k1-p6ÍÁFQ‹ ”Ì˜‹(MÁ �%�nÊLÉ¹’”"*5(²�Ej
•c±L‚‘,XDf.&¦cÚJ¥E0�
´åœÛ\€M™P,ÈÅÌ”¥ƒ2²‹ XÀL&1I›²mÀØˆ\º-a�’s°æØÈÁ„˜LÇZÂ"D¶µIÕX@¦°X®ŒAc0]4¦+‰¹�mEV¢P:§2™Á6·æ`0¦mé6!Ã-‹Ø@·YTm.ƒbÛœÛh›AnŽÈÐ\,5³2“4¥AE±(7’U	©©Î!Š¤\°*‡Z©j©*mAPnÀbµ|+4  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  }     h®ZTR

BASE      RO  xÁA  ƒ°YëñÀ þµ,9¶bƒ"š2Àb+Á E¨ŽLÅe"‘ÀÈ¡2Ð‰™J$¦¥&9°ÊÌ¥–.Š`Î9Œ™±f°µ¸¦’ê˜Á¦FŠK˜$AULƒ˜Ó	ŒÉ2u1mÌ•Œ t.�¦Är)é¶±XY+×ˆ�-eµÂ�©l3²É‚6§–Û–Pà¶ÅdµMYÛr©›.·šÀ€m0Æ6†±M³t‹-™›Jb0Œ¹©l[2‹Íåæ6mÂÆdŒmŒËÅÀ1gÓQÍŠ¨rÓ±¨ms£-çÆ
Ç”
VƒLÓHÙŠRLG’@›‚l‰`«¬ÈÉdsAšMsl±\
cê²m“Z�9]Î¥¬e�ä”tÆ˜e´4bÂÔÍdU,æT)S«„2™ä$!u¹é–³1Ç–0ÈÅ\nZB©Êª(
t›’nMXƒ ò¼4É  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  y     d®ZTR

BASE      NO  xÁA  ƒ°YëñÀ þµ,¹·­­9'a5sc.„taÁ�¬r¬¹¹¢9iâVƒåtDn"aéPG*išÎ©£ˆ,lAŒ„¬ C’X�Á� Õ�!id’`-¦ŽA¢Á¦9Ø(*c”’s¥)¤±ÒM™)9W’R¤@¥E¶±HM¡r,–I0’bƒÈÌÅÄtL[©´&P�¶œs›Ë°)Š¹˜™²tPF6b˜É$&)cS¶› ‘K·%ŒRrÖ™#˜“éXKXÄ‚È¶6©ÈË•1h¦‹Æt%1·±­¡ÈJJçT&3ÂæVÂÆ´-Ý&d¸eè6‹ªÍePl›sm3ÈÍª‹¥fVª’¥4¨(åF²*�"S#Õm2�"W.Ø€Ê¡VªZª*m± Ü€Åª%Ôö™6m  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  <AlignableSequences><Sequence name="34_CMV-F_TSS20210826-027-09501_A03" ID="0" sortOrder="0"/><Sequence name="34_EF1a-R_TSS20210826-027-09501_G07" ID="1" sortOrder="1"/><Sequence name="35_CMV-F_TSS20210826-027-09501_B03" ID="2" use="0" sortOrder="2"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #•<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3636-3645" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6591-6607" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6567-6583" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6660-6681" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6615-6621" color="#ffffff" type="standard"/><Segment range="6622-6639" color="#ffffff" type="standard"/><Segment name="-35" range="6640-6645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3339-3607" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8519" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7728-8519" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8520-8588" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5051" maxFusedRunOn="5051"><Segment range="3666-4328" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4452-5051" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6969-7557" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3115-3326" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5159-5280" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4448" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5051" maxFusedRunOn="5051" detectionMode="exactProteinMatch"><Segment range="4398-4451" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="DRD1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-3074" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>714b9e00-a317-4fe0-ac1f-d017b83959bc</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="7:32:2">2022.10.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  .õ     9   {         {{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷ØïW}Ñ˜wtJ‡˜�{ÈôP…Ä@ú€?^8ÿî'¾‚j- ø(Ûà‚ø/¢5�D9UvM¹ûá~jáøî¾+s¯ë�±çR…ªcØ‰Fû¸
¦�Ðè�1ª¶Ûã‡	ø=²÷<Š�£p|·ª¶ßM¶Ú€þnƒ¯cÐéž«ÔX­í÷~4úÞ¯·>q8«C¸W…û¨	£ê(-†ÛŽw@i•ã”qõaVõN/*¨¶Ømºªî¨þu¨yžš¸ªõ¾+a"DÑÚ�ø8�HpB>w7Ž"×ô†ç>²‚
ðR~0ÒŠ÷C"#~
Vÿ¯»È@´ïvïït6»Ž¸L<ÔŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6  ¿  
ð  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #©<?xml version="1.0"?><Features recycledIDs="0,2" nextValidID="20"><Feature recentID="1" name="CCR1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-2801" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4175" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4778" maxFusedRunOn="4778" detectionMode="exactProteinMatch"><Segment range="4125-4178" color="#cc99b2" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4886-5007" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2842-3053" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6696-7284" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="12" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4179-4778" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="11" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="4778" maxFusedRunOn="4778"><Segment range="3393-4055" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8246" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7455-8246" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8247-8315" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3066-3334" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6342-6348" color="#ffffff" type="standard"/><Segment range="6349-6366" color="#ffffff" type="standard"/><Segment name="-35" range="6367-6372" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6387-6408" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6294-6310" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6318-6334" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3363-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>61a050ee-4b90-47d0-b121-5ae8680fa6f0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="3:48:9">2021.8.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  /®     =   Š         Š{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷ØXà!Ñ=`ïP`èõþaA§a¾ýtâŽ"ˆÞë€øå#ør#¹¸o‹ïß^óÁOíŸŠÚ: áëàôˆ.£cN¢¯b«Ø"½C‹¯Žh4Èy‡ëtûˆ	>øáóÚû�Œæ÷ëçTî; P7‚à6Ø€¾í7’ïŸ�û`Ž/Ðþ;„ê¿xoõ‡ŸŽƒ¾+lúûªð[nwæb»�CöÓbN‹ƒßAÐÐ�A`üXC�ˆBƒÔ�9"ƒˆï¶â‚¿HàþØ?tø=ç;H}QCê:¸ïcˆûm{Ø-‹nc¹Á Do;î?mãàíàQHˆ@>€´CUSüFûK|GD8`mï ôkÑ‘û¸há0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{  ¿  
ÿ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #©<?xml version="1.0"?><Features recycledIDs="0,2" nextValidID="20"><Feature recentID="1" name="CNR2" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-2816" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4190" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4793" maxFusedRunOn="4793" detectionMode="exactProteinMatch"><Segment range="4140-4193" color="#cc99b2" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4901-5022" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2857-3068" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-7299" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="12" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4194-4793" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="11" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="4793" maxFusedRunOn="4793"><Segment range="3408-4070" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8261" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7470-8261" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8262-8330" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3081-3349" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6357-6363" color="#ffffff" type="standard"/><Segment range="6364-6381" color="#ffffff" type="standard"/><Segment name="-35" range="6382-6387" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6402-6423" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6309-6325" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6333-6349" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3378-3387" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>61a050ee-4b90-47d0-b121-5ae8680fa6f0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="3:43:48">2021.8.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="1733..2815" colors="black"/></TopStrand><BottomStrand><ColorRange range="1733..2815" colors="black"/></BottomStrand></StrandColors>  /g     ‘  !Ú        !Ú{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷ØRÆù>�÷ëÍößx~øœ€íXv´œQÛT!Ø6õizýÑZþ¥ëê@¿¯Qaãôä¿MBiÑj�u.îËëPE„vôˆ­�Ù*Ø/…ÿOM8=£KâþÜà7ëp¾_¾â2Ûï{óO¼ãCëçÞ¶ÃOƒ4~ø"Ú¨¹Ó­¡ëÜŠ÷ÍQ“Ír*âµxÜ>úÞ>òOŠûtöÇBvokt?àù”A¢ßCõ ƒ*�† }“Ú!³Èãîøã^5¸¨íªýÝ¡…~~®À-ôÉã®+l‰Œ�‰šîÝ=Ï/6Ó ßÑDÛm½Ü¸A€P�GÔüØ¤)=Gø/†ø Šn8ûŽ�m‚&º€Q…Ôí¥"£k�&;�Ž½òý¶æ;„P~1ÜêðMŠ¿º!CxÍ>àeÓ`ì>7WsWXÓÒ+t4TãmDÜ-F�P—Ø¸‹ÝË�î!0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{  ¿  O  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #Â<?xml version="1.0"?><Features recycledIDs="0,2" nextValidID="20"><Feature recentID="1" name="CNR1" directionality="1" translationMW="52859.41" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-3152" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4526" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5129" maxFusedRunOn="5129" detectionMode="exactProteinMatch"><Segment range="4476-4529" color="#cc99b2" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5237-5358" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3193-3404" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7047-7635" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="12" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4530-5129" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="11" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5129" maxFusedRunOn="5129"><Segment range="3744-4406" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8597" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7806-8597" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8598-8666" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3417-3685" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6693-6699" color="#ffffff" type="standard"/><Segment range="6700-6717" color="#ffffff" type="standard"/><Segment name="-35" range="6718-6723" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6738-6759" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6645-6661" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6669-6685" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3714-3723" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>61a050ee-4b90-47d0-b121-5ae8680fa6f0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="3:40:26">2021.8.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  5Ÿ     �  !Ô        !Ô{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ­…,o‘ƒèÑŸ~¼ßm÷‡ï‰ÈÕ‡kIÅµBƒoV�'¯Ý¯ê^¾¤KúõF0×ÿNKôÑô!v�©×Rîì¾µXAoH€zØ‘"­‚ø_ôôÓƒÚ4¾/íÎ~·àEûàŽ#-¾÷¿4ûÎ4>¾}ël4ø0ƒGï€-ª‹�:Ú½È¯|Õ<×"®+W€€-Ãï­ãï 4ø¯·Olt'fö·Cþ™D-ô?Pò2¨øbÙ=¢<Ž>ï€à^1ã[ŠŽÑÚ¯ÝÚWçáŠàlßLž:â¶È˜ÉØ™®àPíÓÜòóa­2ýM¶ÛÝË„	Ð‡ðô}OÍ�ÚB“Ô‚øo‚¡¶ã€?¸èÖØ"a‹¨]NÚQÂ*6¸Òc¸øá{ß!Ûnc¸A%ãÎ¯Ø«û¢7ŒÓî]6 Áããuw5u�="·@óEN6ÔMÂÔhõ	}‹ˆ½ÑÜ¸ñâ5ã;Â?- 4LÝ³÷KüE( h\ùBÀ%xHbÈžóê¯Ä EòfR4’Ï!rº«5À¨óÑwNëÍ»¾·3óÏçá=½Ï
Ì®<ûóâûáxËÏ.BR¥NÐ±ê/ïèçä{Oïs¨øŽŽ×¹Ë¨«*¸®3ÊtoSˆ|3ç9÷Ø1ÄL>?=�<Û|7ø\2¬N0DÿÔÐ<Ø}uQ7Õþ­?çã„÷!€ç¹÷¬7çý0çëãsÛ]pÃ�õßÍ±œAÃŽ7!:ÓŸ9Ä<<Èl1ëP‚|:ÿtÈn·ÇQ¶óMsß Møßör8 4ë?7ëOŒÇ9çN²=ØÞµ=½ÿM¾4\ ÿb³ëÏ2�Ó]ñ8²ŸMÏ­GÿlúÏFüÎÒËÖ¸òóÁü;ßìÄRë—croÃù‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�…m~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰´¥è©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.¡íXbÖ’æÑNn¿¯ Goâ¥š•ÓH¯Õà+¦oèî_B�¯‹íÐ\RþTWH4ô*OðZ¾ª�?h&ªÿ&ÿô"ãCµDÓL­TÆü�~¿Èü/ós�ÀÀ3ÁðLü;8ãÿ“ –�¿€  ‹øfæ]EY™™e)·%$]…eÖe¦É„¥n•KrUmŒ¶¨{s)’µ[Hw§Í¡ÔÜûp½L1á¾V7Lpk3š‘Q5šµ62Ö©Ín®@¥¹8ÛA¶l°.ª¼;KlýNæà6ÀQOÈú¯ÌB…0Ø£Äxã�¡Êsr§Øa¬ƒØÜú—^++Cß€gÒ²Ì:¯t¯OÅ3m5ü]gÃ<�#Â#F–0Œ3€fœ´Ä	¾<ÑX�ŸòŸÃ3 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶  ¿  I  q      \®ZTR

BASE      F,  xÁA  ƒ°YãúÀ þµ,9M1D\³Â„Í†4·™mÌe•lm„ µN¨	Ð”676R�¹0t›Î�Z´¹$…Í¥ˆƒåt‘ s[Z[
ƒrUÉ1¢‰¦ŒÆŠ¹ÄXM Û¬ié\$0‰Ô%Ä€ÆšÍÈa¬µÔ*rÓ,uÎ¢¢)–ÃrnsÂdT	3¡\ƒa¤Žj·ØL³fµ Z ª„™ \Û Ñ EÑH©0¥ØP©†M2Æ¬Ù­Ñbk›VABLªÌ±­&¡2�A9–4ÖÖRZ,m¹h6×rk„°Ür&RkŽÜi‹ªtÐ€FmJ,'U`–µQ�i•BV,Æš•D¦T›­ #¤qY-QÜj(Å@j€9—KsƒmQk«ÕÜ kIÉ€˜T´ ‚M \Íìë,§  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  ^     I®ZTR

BASE      3è  xÁA  ƒ°YëñÀ þµ,9¨Ê(¢),V‚ 6ŠP™ŠËD"�‘Ce 2”(H�¡¸¨@ (…j9]Däª¢ÄpV�¶9¨ÐFa0¶16‚@d£*DËåHqØQ£“`ÛØØPu³(ÆÂP7‰c�n.K5K'›KW°uÂ ×„ÊFm1ç(¦hl¸ÜÜÌÂ(˜mÒ2‚¹e,(c‹M±4¤@WVcm cm±¦ƒˆ1Æ,„¨\mL¬´sÁØ �bÛ¶tc£8­€P³-æÖ$—Íˆ™XJÈ5H™K7ˆR6�pÔœS�ƒ¹ÉVa¶­æÚ¶ÊØbÎ¤JqsÓ¹5µ”Ád"£r‹²-ÈM	bncE±EkT›Ã>(…Þ  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>   ¿<AlignableSequences><Sequence name="4_CMV-F_TSS20210421-027-02776_D09" ID="0" sortOrder="0"/><Sequence name="4_EF1a-R_TSS20210421-027-02776-01_D11" ID="1" sortOrder="1"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  $_<?xml version="1.0"?><Features recycledIDs="2" nextValidID="20"><Feature recentID="1" name="CNR1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1728-3146" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4520" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5123" maxFusedRunOn="5123" detectionMode="exactProteinMatch"><Segment range="4470-4523" color="#cc99b2" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5231-5352" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3187-3398" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7041-7629" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="12" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4524-5123" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="11" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5123" maxFusedRunOn="5123"><Segment range="3738-4400" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8591" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7800-8591" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8592-8660" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3411-3679" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6687-6693" color="#ffffff" type="standard"/><Segment range="6694-6711" color="#ffffff" type="standard"/><Segment name="-35" range="6712-6717" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6732-6753" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6639-6655" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6663-6679" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3708-3717" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1710-1727" color="#ffff99" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>61a050ee-4b90-47d0-b121-5ae8680fa6f0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="3:37:28">2021.8.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  :8     Ð  "Õ        "Õ{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬Z•°o=öõWeECðÏk›ÎÎMxÙC˜QÏà¯�u£ªtcvlBméÎzÅbò° ÑNa\f…�[t[,˜Ò{ºÖº˜ðŠ2š›£J3ó©–!hít˜«4ùò¬«ÕP
5Uª#UTïÖÛÕVšm�®UÁ§Ð´±‰Ü¬·nÛŸ¿
¥…œjˆôKëÔu£xm…ï¸ž�•³¸ûd^>2®Í�Doš¨5µm¯xÆ©H¢½¯mÂ¨*yÞÁš†b¬K.–&F¡EŠ¸A>´uÕ#8à�_æ»ë¬õS{†‡Vq©¹jw£®3¿îå,L(õ¯n=	´5`;xG�´æ¸ißÄ a•ð3	RŠöª…1Bˆ�ŸW8¥µD1xˆ„Bù†˜¼)‰]o1õÏ¡¡Ô9ÚàGdéàQÅÞºÛ(|øRîRuC›Büáhöèï]ÿ]ºÇ4"Í¯ÇsÂ¯Ï<¢Š MÁQÆQ iË=-IuóRŒÁ¢* qIñPº|{ÔUmí•Q@ÁQ³È”ÃÍA¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹�h{Vµ¤¹´S›‚ïëÀQÛø©f¥tÒ+õx
é›ú;‚×Ð£@«âût¿•Ò=
“ü‡�¯ª¤Ú	ƒª¿É¿ý¸ÐíQ4ÄS+D1¿#ß¯ò?‹üÜãÃ°0 Lð|ÿÎ8ÿäÀ%§oà¨  âþ¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  Ð  J  q      \®ZTR

BASE      F,  xÁA  ƒ°YãúÀ þµ,9M1D\³Â„Í†4·™mÌe•lm„ µN¨	Ð”676R�¹0t›Î�Z´¹$…Í¥ˆƒåt‘ s[Z[
ƒrUÉ1¢‰¦ŒÆŠ¹ÄXM Û¬ié\$0‰Ô%Ä€ÆšÍÈa¬µÔ*rÓ,uÎ¢¢)–ÃrnsÂdT	3¡\ƒa¤Žj·ØL³fµ Z ª„™ \Û Ñ EÑH©0¥ØP©†M2Æ¬Ù­Ñbk›VABLªÌ±­&¡2�A9–4ÖÖRZ,m¹h6×rk„°Ür&RkŽÜi‹ªtÐ€FmJ,'U`–µQ�i•BV,Æš•D¦T›­ #¤qY-QÜj(Å@j€9—KsƒmQk«ÕÜ kIÉ€˜T´ ‚M \Íìë,§  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  ^     I®ZTR

BASE      3è  xÁA  ƒ°YëñÀ þµ,9¨Ê(¢),V‚ 6ŠP™ŠËD"�‘Ce 2”(H�¡¸¨@ (…j9]Däª¢ÄpV�¶9¨ÐFa0¶16‚@d£*DËåHqØQ£“`ÛØØPu³(ÆÂP7‰c�n.K5K'›KW°uÂ ×„ÊFm1ç(¦hl¸ÜÜÌÂ(˜mÒ2‚¹e,(c‹M±4¤@WVcm cm±¦ƒˆ1Æ,„¨\mL¬´sÁØ �bÛ¶tc£8­€P³-æÖ$—Íˆ™XJÈ5H™K7ˆR6�pÔœS�ƒ¹ÉVa¶­æÚ¶ÊØbÎ¤JqsÓ¹5µ”Ád"£r‹²-ÈM	bncE±EkT›Ã>(…Þ  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>   ¿<AlignableSequences><Sequence name="4_CMV-F_TSS20210421-027-02776_D09" ID="0" sortOrder="0"/><Sequence name="4_EF1a-R_TSS20210421-027-02776-01_D11" ID="1" sortOrder="1"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  (¸<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4777" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5380" maxFusedRunOn="5380" detectionMode="exactProteinMatch"><Segment range="4727-4780" color="#cc99b2" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5488-5609" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3444-3655" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7298-7886" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="12" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4781-5380" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="11" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5380" maxFusedRunOn="5380"><Segment range="3995-4657" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8848" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8057-8848" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8849-8917" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3668-3936" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6944-6950" color="#ffffff" type="standard"/><Segment range="6951-6968" color="#ffffff" type="standard"/><Segment name="-35" range="6969-6974" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6989-7010" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6896-6912" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6920-6936" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3965-3974" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="2" name="luciferase" directionality="1" translationMW="60644.17" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1751-3403" color="#ffcc00" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;enhanced&amp;nbsp;&lt;i&gt;luc+&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;luc+&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1745-1754" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1710-1744" color="#ffff99" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>61a050ee-4b90-47d0-b121-5ae8680fa6f0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="3:37:7">2021.8.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  4Ž     Î  "Ï        "Ï{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬Z•°o`QÏUvTT?ö¹¼àQ�|àD×�”9…œþ
øZ:§F7fÄ vÞœá'¬V/+
æÆ`8YÕ·E²É�!W»­k©� 3)©°J4£?1qÚ™arŽ×I€Ú³OŸ ŠÊ½U £UZ¡r5UNým½Ui¦Øå\}«K�ÊËví¹ûðªXYÆ¨�D¾±�GZ7†Ø^û�¹à¸ùP‹;�¶Eãã!ìØôFùªƒ[VÚðgŒj”Š+ÚöÜ*�b§�ì¨f jÄ²ébdjQ8«„ëA§Q­R3Žõþk¾ºÏU7¸hug›–§qZ:àƒ;þîPRÀÂ�Q
öãÐ›CPf·„yÛNk†�ü@_0°•(¯j¨S(�¸ðs€Ê[TCˆˆD/˜i‹Â˜•ÖðS\úA�ƒ�®vNžÅ]ë­²‡Ï….á¥'T9´ ïÎ�onŽõßõÛ¬sB,Ðºüq‡<!züóÊ(ªÜaÁU²œ³ÒÔ—_1U(ÌJ"¢Ÿ§ÁW±ÍEVÑ¾ÙPõ<‰L<ÔŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6  Ð  D   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  (<?xml version="1.0"?><Features recycledIDs="10" nextValidID="20"><Feature recentID="19" name="luciferase" directionality="1" translationMW="60644.17" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1745-3397" color="#ffcc00" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;luc+&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;enhanced&amp;nbsp;&lt;i&gt;luc+&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1745-1748" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="17" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3959-3968" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6914-6930" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6890-6906" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6983-7004" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6938-6944" color="#ffffff" type="standard"/><Segment range="6945-6962" color="#ffffff" type="standard"/><Segment name="-35" range="6963-6968" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3662-3930" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8842" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8051-8842" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8843-8911" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5374" maxFusedRunOn="5374"><Segment range="3989-4651" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4775-5374" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7292-7880" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3438-3649" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5482-5603" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4771" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5374" maxFusedRunOn="5374" detectionMode="exactProteinMatch"><Segment range="4721-4774" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ©<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:36:2">2021.4.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  -ö      )  Z         Z{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬Z•°o0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2306-2315" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5261-5277" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5237-5253" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5330-5351" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5285-5291" color="#ffffff" type="standard"/><Segment range="5292-5309" color="#ffffff" type="standard"/><Segment name="-35" range="5310-5315" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2009-2277" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1710-1752" color="#ffff99" type="standard"/></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7189" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6398-7189" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7190-7258" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3721" maxFusedRunOn="3721"><Segment range="2336-2998" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3122-3721" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5639-6227" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1785-1996" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3829-3950" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3118" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3721" maxFusedRunOn="3721" detectionMode="exactProteinMatch"><Segment range="3068-3121" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ª<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:35:53">2021.4.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ìý7zXZ  æÖ´F !   t/å£á7î©] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷¿Üµ¡&õý{ÍD¹ÜŠ"XÁBOÎò%‚õ‚{<-¥!`à¯œ¢¶ã×› áD×´¸¹eŸ"vš¬©�J³‘M ªœé˜Ú¸nMà¥¡'Xÿ §t@,ï6qA'Ì¥5¬_wT·g€ÑûDYö@2¸Îõ%RmÛ%ØKfBå< OÓŽ CÖÏ?V¢€AX|8° Ên=nÉ�ì|øoŽóÕ¯aºó<^wÑ$�hù¡¼|=À&ï=*l§O*-BP¡ÔWùþª�žr2Åô…Ú¡'æRŽÕµ!ú¢·âöÏ‚'Ãü¼~Që�söZb;H2•°±g:E³0£æû·D6’ª‘—Wd^/ñ==<KW¤tÌ%d;?É´»R”H·=O‚uÛ½ÝÚLð ðå >�iHƒ€e¢$MÂ~BK‹™®10Þ¼ÇÖ’ÙÒPª~ ;Á
„jÐ·×“âS‡bm†[@	An‡]ñ—èU&[_„½6y"€3D!”M„5iÖHW§6ßµUË\ó£ .]k5%ì¤xqÊ½=<aû�¯O ÔÕQÀ‚Y“ôO±¢õy²ÜT73ó¹ÖšÜ,ÔÐ¤Æ+µE2XsçÙ vÚ:6&oï#e¯OÍ• Ø5\¼5™¤±©ìLW.>ïžmæX©ï÷:ÑYŸFºwÿt™z^YF…€«S¥]°ÚÇä³‰O¯°�ñ.þéõfQÙ:ˆ•¯%¥ù¢‹ÎI+2ø;yg©®ãqÂ‹W$æ©Ld™¯‚ò4eaÏ†¬j°ªtÀ-àµaª¸±yù!Tö@ó"B)ôAS§pÿ›) Ú®Û‘%Ê"ö~ùxwkÜºÇvhjmòxôGÂKÇÞ}´9&»LOy§^‹p§¸Ï´k.hg=Æèµ«©íAÀS§«]ñ–„‰18ã&€x§mü¢·`\‹¾U†b1IÔuŽÆ]æ¬y}uÝuÛXí
`’ñ:—âœ«žl´I(ð.ƒÿHŸ÷¿Uð½ÖpgP–Ïÿq'ºUà²÷åÆ©ï'éøeNZÝRšÂ�Gåx%Æ‰N¤h¶âž‰ì-©·ò¯Íbt™dý2äãn�æÒcÍ¥Øþápòº6‘èV\†´ïæ[ÜÅAßÀå ‘–ñQ˜­›'…ÿ­ÕmhÆ"ù–DïÓ2átMõØËof÷”â<°—\_†v„âžÕ’¨:QxŽ^h¢+¿÷í¿Ç6¶!S?Î9yEH#à¼8 ê$ø¤Èg‰{#6V2"ï¤Ä=%�kÔ<²EÖ:˜¬�ù]&Åýmàÿž£¹øI)sÀhœWþ`ýªT;<Ý2éE9·Œü@Ôx¡íüÂXá¿zw!e€;Brgc­A"—“×†ÀbBw1Ž¤1FÜÇõ:Ë³ë‹BÓ‹{•OÑäcˆ¬Ú½üŽ"Žs éò}'¦µ yúz1yDL’¬¯gþµœÚÉ'|h^§Áy$X¹ÔB/£>™¤Ã¾öÓ‘¶mt;F¿¹±±u`güA�…õv'[wöwoÌøCâVv¯„@·³ƒ3ù×Õ[hþqˆöÿpBža¥PàQeÝîé�ÔƒÌÌsçÎ…b‹d»=™Îóþ÷1QšèrÛÃ¶H~¢ÊÏO]îÔ³›=	�Ðèƒv¹À‡†°D8õ-2œí}åW:RÌiÉ÷ž+ù±�º=|ç‡M[TÍòàØ8ª n8xwHæ”êÜƒ[,¨–ž€•åEÆo)ì~KU£)dÑþ^¸¼:åK]*…{êÏæÄb¦…hM0Ç“›˜ÑXqÍð,:Ýß¨hhT#„00hU_ø+·tÎÓž¤ð´‚—·šÒå¢\KeØíëèÅ°¥LÔ¤�÷‘BÑPÓº	AðùúÝ “òDýmÃã²LXRÄá›?=ÌÆ2&Á�¥H¦‡4CfV]w´�óW&ê%øª§!ÌKDUÚ4©Á#TQà‚EM—tð=uûŒ±Ò¢¸Ò—Ø4€E9{,ð‚ÏòæÄéL¢³løí�tˆî–D`�xÛ¢×>Î�v éfÎ(WâŽJŽn6v¸äKûÂ(º,\’1§%¿x:Ê‹’¬ÃaÐvÃ‡WëB®¥ÂyŽÅ3„ …{:Ü&·¾Eq°ªÎc¦Ž†lNÄæÊb~ä‘×].ò¿‡¦Ÿƒ<6½EÔîq³¤gQÈa”¹…F<�ü&>ìÌk›HóÅ�~SáRöjœÍQ3ëÿÎnºu²�ÍÅô®k!WùÑ˜¥8!RIN¯«s€k�‚>øJ0SãZFDt78{˜sŸöeŽ½Ó#µøÃóyè¬´¹§¡Q^�8Ü©Á¬#Vì™ƒ'¼—CwA€ÿFM}2åÓMB¤A¥4ÒÔcÇ{óýà/—‚&NƒÁíhÉw
Uózw€\Øôð�!�¤ÓMRà	]zûÅÖÍhÜ dC±$rFûz,à?Â	5þžõÁå2| ˆŠ�î>ºP1%v›�ÃÊ2»gSK¦ÜÊÎÙ7}y“¸ëA& 
ÝdcynÂQîXÅaˆ%r3ÃKêÛÀòØkczn¨	~ºxÃùøÌß�x–íšwuö5Ë]†ÞŽÌ ½ÌVí¥„ÓHàÑº.YSItæCoôÃ>DÖ}¾Æ!_yvXÙkéx\+¬¥W	àÞ¸xÃt!,•­ÆoŠ9è–¼Ù»-Ü‚öêyÔX¼”¼oö P§—�]xThƒ’ñù¨sª·œƒÌZöHÄ*‚PËq¼9©¹·B�ø‘Úù&9µ'Ô�Øpën 89LF¢/LÐ�ûÈ�­ñ]“µ®åª¤rÛ×¨hðLä°&ÖÈÃþw4—Î…~Ðç¤%ÅÚáàc¶DÝ§$¡µŠN$qà4Ÿ²V¤Bá« Ælú'µ
”[Ô.:ãWiº&Ô¦FTúÕº³Ã=ŒH÷u©é %fŒEÒÓt c YŽÕðMÐ¯?�z“›*]OB"YG®V:�¹ÚGá	Çól:¿’@ÚËa�-µvnO\[ÒK G3 «®F·…ã_ô,eÐð¥^´¸èô5)„bÌ„7M¢öÓ‘UÍcòQO×V—UÖS3ˆKœ^3#ä“/q="Õ¼rK…ÌÚ1�ˆÔ.tõË8“I,ö4m¹BÈ�î¤«EÌ�†Áå\6Yy«=€ÜÃÑrv€*2¦\�w3TŠ½�©üÈs>Ú=e’=î:ÉÖÇEöæ°ÑwÄù1ZgìGYÿcô¹u�Ð¢™9çÕÅÁ”ÀÂÐJÃŸ½;&p¼uŸÍt`µè¨VªÏ0'¶Ýa�5‡"•ŒL°u!Šëk®x$ÊÈE)yÄßF{Ë³Ã%7å#)¿¢ƒŒÙÓ€I�,�jÆ([Ž” ¤—çÇì�”ðÉ]jó¬<ƒcçèc‘ôOi¨Ö,^¯ï"([�ò'ûÁVð¥ž'Hnd}xt�®ÂáÒO0i1RaÍEyVšmøut<|X“(%¬ÈààN”ço @ù=¦›9jÆJG6t)Bd«rŒ†˜L>æÈ¹ÍËEá^í\îÓã_%IycÔA�S/º;1Ý¡zÓª#bHý6iË”ïØÏ…¢]n†¹N„°< “àmfíŽÃÕU
½ñt¯F¡ÞÊ±¿®‚ Š’�9¯ZcEâ(;)Ôc#r{Ôd°c¤Æ–ýÚ'Ê�VCeå	éeO�åÊ|õÒïŽIÎ5 ¢´sNRûYÏL÷O•b¡$[½Ÿ¿q´î?+çùyï€ÞÕ¥ÌöžW
†n}�Ê”7"s>—?¹À Ê2}» TPVí¢¹Í‡­�>®§~ ìïúX<ñqßgÃª›/nåûÁÂz‹isß&:'“Wm Ý�#€}½Ýc7q}°’‹\]).-‚Ï“f«ô7Ø{á#¯À.RŠ„È¸ZáFxn K7¤ŽWC‹â`	é¶G5^¨7Á�)”ÑÆüÁu‰ˆ½R5ýAË&�§¨áåÿ.,�ãÔ9¶ ¼w§³Ê×ó·´ùKÅ˜œPž5BÕÖ¬DðÅ~6ÞÚrV±`hÔ7%L`:¬’µÃl0tÃíû7¹¡Ý®®ª%tçœÿY,>nEÁ•Œ@š¿^€d’t@Ùž´kV«È`WáÒ|‹4F€«þ‰¶•~Â �w˜kÆÙ dÞîï‚Žñƒä´ŸA‚©ëéÚôe´§K-ESüˆÌþ( y¥Z–îGS;Ô¼<}µzŒMš÷Ø›W4Öq@–jlg$sRUÃÆ5â$ƒšê±Ë8�«ÕP‡,SÇïQó8·pÜñBN[Ñl@Tg”ºµéF—ò}PÅÏ�Ò¤Õt±FL�ýpm_ŸPÿÜO0—…XŒI)Î“1IS`ýæ×Kv&�™kyR¶v&î ž)ÆÒb„Ïi[_¨»Ýâ²¦V‘3Êo	ÃM}¿ßGY÷Þ(cÑùÊ€p]º„TÈ-¦l~'[ÂwÐÇ"‹±÷tÃcsg!OKtn€½êl{ú”€Kñ¼Äá=rŽþ¹‰e™Æ}r¢òÑüH^zÏäþ)BÚò}ÒäpÁDXF¤ê`ŽÂ.ø×Åóàãé÷é|p•×ÌÈv	ÅÞ* d©5djÚ"Ô"ÈŠVu2Bj¼;8´¾üïÞÍTô$åÍ+ácºfF	¯ÛËLÇáÜÂ‘è[l9N¢sH:©u¶‡ž¯ÎßöbÍ.Ö¡ðøûÊÏ§ÚÑ¦Yðüø<yS…\Ãrˆ¿[ÿÌ84ˆÊcù[’×Ù&=m‡;zö^i3°øÆg?5d\ :…M¿ÄY<WI‡êA·`ìn+6jÇl›ÿj³‹3âHÏ\hl…“>c*:Äæ;Ñlî˜µhHx¦Ò•†í�Ãššê»0Êq‰
/˜�X½Gã=ŸÑôø…Â�–Ã€!ð;“æe	ž62gakÀ84Q; %õ:äP£±×%&5÷Ur“0)ûæNêLU}Ú|¸NöÍ…$‚I’á¨à–>Xòµ@Dö\xŸ&T¨ˆ+OÐ¬ºHk=0€›ñèÏ>…�õ,œÝÆQJ¾Bu6¸Œgù'ÊiŠ¿U3n¢ò9äF¸oœ‹×EI#û�fµv-ý ·Q–PâÇ*ß§mÝ;m‘ùº.éŽTþ$ÛÆ*N’Ýž¾³;ç‡ðc²?V3N„Þ^¶t£BRO¢ŸŽ‹¿ªDøAœ›ô–à—auGd>U¬œS7Œd\ì/óXÛé”ZTó_]¥dë/ËÓSˆÕÊYLãÊÁ4g…òUÔ`ãi+ë]îü› ¾rÃwBácîOŸ	ƒÂÞÎ™�|1Ñt¿’}Àƒ^ém¹2{Y—�FLÕ¤œ!Žƒpmµ'§",I&ßa¬ÃñŸ¿Ü�}È·›´-Þ!+€Ä
J®Šè÷%²–ÒáyËÑÄ*3é>ýÕ˜%`ÀÄuò7�œE‰:›�aáÀúDª½ÑÆÔµ=Ñ–Ö5ý¢�d œQÇ¾ƒQ¦â—Êˆe(–NÙ™áRÈ·Í�lcTjóOu¿—^´è®ƒF;Qf1gŸ\Þí´øhdïÓ!Ñ}¯Óñ†³ÕQœn”æ ›!>[‹ŒÊ—±½TÎ€œîïuÂ>Š‚VÛ*¦Ïˆ,ÜØûù� .aÞü!R*j.LÞ•ÏN�³DÄYx ¼óˆ÷¼¬ô×Û)Àý£B¢œ§šw3•y„µ�
Ï¶S„ï@[¤gñ!Æ›ÖŒkNùŽrÃøã[AÉN™”º§tB›4zäõ­%@ÄVÇð<&ÄÔ�øß-¯X_C�ôÜââœº:ˆ¥œ€öj^ÊéAXZUdÖZÝd­¢ˆ{»š×0ØžäÌ5€³ŠgŠ¹«ì€}i#ó(~M­4>ï¯NÂíÑi:¾ÛÍßÊÌôãà˜4á’çár¢/|õÍÅ3ù>_ªªM*ÝÆ"Ù1 Z,P"É\À\8×`$E7ò‹´í7ªê³f‘„Þqhœ„çÁÓ3/]5n0/#•= âZ¾BC0rØö¿13çõ�"|™íŠ†÷gE¨é¯Ûd¢Y“[¸ÇêðUi³r­2ó8akA“ÍÅ#Þ(æ%€X5=–LšZ7ëÅžWS¸¹S†¢l0~Îª��>V"*ÉÝw3.«i‰œ°fgŒÒÊqê»¸aÁè“¤{ÂÇ¬ÂÅ,bC!LVÉÇW¼ìBÊ*t3
á«„vqqCX¾~ù°û‚M£#Tÿàc>è‘L°®HCA‚hb!Æ$îtß­˜~Mú¾¿ÑØÙÑâ�0Û^¥B‡óïšø—ZÛÁ£Œ,�6[ÿý½þP®Ð7½Ðão;ÔÀƒÙŽ©�óŒî—øË©ÔæwÞQ0û)Ô†~p.eQDò|–Û–ÌâÓZŒîÀ2·ÿ>�Ô´V¡”Qw’5.ÏéMºZdõà	¼ÎSfs°dI®Ê‘õÞÍÜÈïGÏ�ò+×ž8ÙnïGN±c>H›Ö¿      œÀÊÈ3Ýžã Å'ïï 9«_¹±Ägû    YZ  v      a®ZTR

BASE      KF  xÁA  ƒ°YëñÀ þµ,9–élkÎIXÍs!¤s†d•ƒ`ÍÍÍI·B,§#rc	K‡:RIÓtNEdab$d’Ä‚†©nI#“k1-p6ÍÁFQ‹ ”Ì˜‹(MÁ �%�nÊLÉ¹’”"*5(²�Ej
•c±L‚‘,XDf.&¦cÚJ¥E0�
´åœÛ\€M™P,ÈÅÌ”¥ƒ2²‹ XÀL&1I›²mÀØˆ\º-a�’s°æØÈÁ„˜LÇZÂ"D¶µIÕX@¦°X®ŒAc0]4¦+‰¹�mEV¢P:§2™Á6·æ`0¦mé6!Ã-‹Ø@·YTm.ƒbÛœÛh›AnŽÈÐ\,5³2“4¥AE±(7’U	©©Î!Š¤\°*‡Z©j©*mAPnÀbµ|+4  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  }     h®ZTR

BASE      RO  xÁA  ƒ°YëñÀ þµ,9¶bƒ"š2Àb+Á E¨ŽLÅe"‘ÀÈ¡2Ð‰™J$¦¥&9°ÊÌ¥–.Š`Î9Œ™±f°µ¸¦’ê˜Á¦FŠK˜$AULƒ˜Ó	ŒÉ2u1mÌ•Œ t.�¦Är)é¶±XY+×ˆ�-eµÂ�©l3²É‚6§–Û–Pà¶ÅdµMYÛr©›.·šÀ€m0Æ6†±M³t‹-™›Jb0Œ¹©l[2‹Íåæ6mÂÆdŒmŒËÅÀ1gÓQÍŠ¨rÓ±¨ms£-çÆ
Ç”
VƒLÓHÙŠRLG’@›‚l‰`«¬ÈÉdsAšMsl±\
cê²m“Z�9]Î¥¬e�ä”tÆ˜e´4bÂÔÍdU,æT)S«„2™ä$!u¹é–³1Ç–0ÈÅ\nZB©Êª(
t›’nMXƒ ò¼4É  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  y     d®ZTR

BASE      NO  xÁA  ƒ°YëñÀ þµ,¹·­­9'a5sc.„taÁ�¬r¬¹¹¢9iâVƒåtDn"aéPG*išÎ©£ˆ,lAŒ„¬ C’X�Á� Õ�!id’`-¦ŽA¢Á¦9Ø(*c”’s¥)¤±ÒM™)9W’R¤@¥E¶±HM¡r,–I0’bƒÈÌÅÄtL[©´&P�¶œs›Ë°)Š¹˜™²tPF6b˜É$&)cS¶› ‘K·%ŒRrÖ™#˜“éXKXÄ‚È¶6©ÈË•1h¦‹Æt%1·±­¡ÈJJçT&3ÂæVÂÆ´-Ý&d¸eè6‹ªÍePl›sm3ÈÍª‹¥fVª’¥4¨(åF²*�"S#Õm2�"W.Ø€Ê¡VªZª*m± Ü€Åª%Ôö™6m  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  o<AlignableSequences trimStringency="Medium"><Sequence name="34_CMV-F_TSS20210826-027-09501_A03" ID="0" sortOrder="0" trimmedRange="15..1089"/><Sequence name="34_EF1a-R_TSS20210826-027-09501_G07" ID="1" sortOrder="1" trimmedRange="4..1097"/><Sequence name="35_CMV-F_TSS20210826-027-09501_B03" ID="2" use="0" sortOrder="2" trimmedRange="15..1099"/></AlignableSequences>  ¬‹     ÿ BC N srôe”```pðpáó³2Ô3ãö·²*Í+Î/*IMábÊqqQjZjQj^r*C�" çÎ²Z6   ‹     ÿ BC @í—?®EÆ÷YéV#uZåŠÌ "$�«UHM‚8‚3R�)UP!Gà(�	qäæë·oõðÊ™#kKõÌlÿùªGÛ¿úþúøt?£}òú‡ú9î¿ÿèôF<;ý³›»Ó©¢yõí¹}q:·?=´ÏÚK¿ßžyhÿ8=ŽûEè*‡êô•-êðÖ‡êàÎuF¦²Ó¡­û¡ó˜Ú}wµêQˆ=¸[5›±Ãpë9’)+9|†�ËÂt.f…†˜GÈÙÉ’•8ÆâbQ©¦ä"w‰)"˜–Ç¢`±b´LTsÆL1–´Å)«�‡Å”Tò¾¦céý^ 0¢Ó\VÛêaÒ…†±ÑZi]™©ÍÆ	…ê-b"	�C¶ïLk6!,X±z©\Ëd-Á5!
z¸·ZdéžÜYí}b8ÄÑËÌ¬�‰tŠ^¥V².µ!o¥–^mPNã´ÖŒfs\´·JéËa7G‰ªCËòÌ¢…¥¨)ú>ŠòÅfD"sÁ¦œŽìRkPæÄv™-\­î´e¦:Î¨¶ÒÌö%Å…1 z³?}‹[ÜâïO?9Sì‹OÏ„{zúìôãá¾<Ù±ø?d{yÕ^w!Þ³Ëû»7ûýzõûïW¼<¿xò0þ›G2nÅl‚@†ÓÜÄ(Ú
ÎüÍ‹²y�sÖ6.øL‹Üï¸�aA»Sa4t-}þïY±ÞÆŠõÈ
q¼Ôd`´X+×²ÄhàbÓ3
o^Áyô=}ã:“*{6¤PŸ:
: ¦Ð±tuê…ºOE±�=�¢ÆUM¬.Ö‚ºX@1A¥¡ŠÔ¢¿ªm4£diuoH™áL“7ï=Tº’èpÔ$Š†ñI¼ÖRÆ¢YñmÌAM=FÂŒ(ŒtÎŠI‰3n(6[cl�­)¨ìù¿ï?Ö-nq‹%þ¼2‚÷×˜üêÞ¾üîŒ«¿Oo7‚¯.ø»{{»ÿÀ•Pz4~…©OÙG=�(pnQq·¢‰ÓNÚ(9¸âlVœ¾µŠL
Vqü<Pï&Ë@Vƒ#áø†Lp-öt†Q0‰²fð:¼-˜fšS[ÊE<Ãqâ§ÉÚR<×Ãè}Â�‚«°„­ÃÒ€C SxG Kˆ7BàrÀˆ p-ºJv¤ÍÃ�qàm¬®€ FeÐdß
;�eÈ‡úê½ŒÍò  Wì
æ˜“8o=£Qw� +�}Û]˜Ò£I˜¦¶Talù lžX•cÌÙcLÙÞy6Ú.™ZÉIŠÝ†t,×°…Ú—ÃÎâùh¶—
(T�w\ðQÖ0õnkmãnR%‘À7¼É/ãm¨îaÞcóžycž0ÎÎ¯_œß&yyßˆ[ÜâJü†¹è  ‹     ÿ BC             !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  )<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3636-3645" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6591-6607" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6567-6583" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6660-6681" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6615-6621" color="#ffffff" type="standard"/><Segment range="6622-6639" color="#ffffff" type="standard"/><Segment name="-35" range="6640-6645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3339-3607" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8519" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7728-8519" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8520-8588" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5051" maxFusedRunOn="5051"><Segment range="3666-4328" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"/></Q></Feature><Feature recentID="9" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4452-5051" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="10" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6969-7557" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3115-3326" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5159-5280" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4448" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5051" maxFusedRunOn="5051" detectionMode="exactProteinMatch"><Segment range="4398-4451" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EGRGSLLTCGDVEENPGP"/></Q></Feature><Feature recentID="17" name="DRD1" directionality="1" translationMW="49294.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-3074" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISSSVISFYIPVAIMIVTYTRIYRIAQKQIRRIAALERAAVHAKNCQTTTGNGKPVECSQPESSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFILNCILPFCGSGETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRLCPATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT*"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  Å<Notes>
<UUID>621042ef-d05d-4f6e-82fc-004cbaa752b3</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac-CMV-DRD1(human)(1å�Œä¹‰çª�å�˜)-EF1a-CopGFP-T2A-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="1:36:18">2025.9.4</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      DO  P              E              ÿÿÿÿYô? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
